Journal: Cardiovascular research

Article Title: Novel technique for the simultaneous isolation of cardiac fibroblasts and epicardial stromal cells from the infarcted murine heart.

doi: 10.1093/cvr/cvz193

Figure Lengend Snippet: Figure 5 Immunofluorescence staining of selected proteins in cryosections obtained from hearts 5 days post-MI. (A) Immunostaining of Tenascin C (Tnc) and Troponin I (TnI). (B) Immunostaining of Tnc and Dermokine (Dmkn). (C) Immunostaining of Vimentin (Vim) and Cytokeratin (Krt). (D) Immunostaining of Tnc and Lipocalin2 (Lcn2). Lcn2 labels besides epicardial cells also cells of the vasculature (arrow). (E) Immunostaining of Tnc and aquaporin-1 (Aqp1). (F) Immunostaining of Tnc and Periostin (Postn). All sections were stained with DAPI to visualize nuclei. Dotted line helps to distinguish between epicardial/sub- epicardial tissue and myocardium. Scale bar: 50mm.

Article Snippet: Samples were washed three times with PBS for 3 min each and blocked with blocking solution containing 10% normal goat serum (Jackson ImmunoResearch, Cambridgeshire, UK) in 0.2% saponin for 1 h. After blocking, immunostaining was performed with the following antibodies: WT-1 (Santa Cruz Biotechnology, Dallas, TX, USA), CD44 (Biolegend), CD105 (Biolegend), CD90.2 (Miltenyi Biotec), Tenascin C (IBL International, Hamburg, Germany), Vimentin (Abcam), Pan-cytokeratin (Abcam), Aquaporin-1 (Abcam), Periostin (Origene technologies, Rockville, MD, USA), Lipocalin 2 (Abcam), Cthrc1 (Invitrogen, Carlsbad, CA, USA), Cemip (MyBioSource Inc., San Diego, CA, USA), Troponin I (Cell Signaling Technology, Danvers, MA, USA), and Dermokine (Abcam).

Techniques: Immunofluorescence, Staining, Immunostaining

Journal: Matrix Biology Plus

Article Title: Periostin modulates extracellular matrix behavior in tendons

doi: 10.1016/j.mbplus.2022.100124

Figure Lengend Snippet: Influence of periostin on mechanical properties within the intact and injured Achilles tendons. A) Ultimate load was reduced within intact Postn +/- tendons compared to intact WT tendons. Within the day 28 healing tendon, load was significantly lower in Postn −/− samples compared to WT tendons. No other differences in ultimate load were noted. B) Ultimate stress was significantly reduced in intact Postn +/- tendons compared to the intact Postn −/− tendons. No other differences in ultimate stress were noted. C) Ultimate strain was reduced in Postn −/− and Postn +/- tendons 28 days post-injury compared to WT injured tendons. No significant differences were found in D) stiffness, E) Young’s modulus, or F) cross sectional area. G) Overview of mechanical properties of Achilles tendon. Data are results of two-way ANOVA. *indicates significant difference between genotype within the day collected of given metric. Significant results in genotype (based on p ≤ 0.05) were further examined via Tukey’s post-hoc analysis, and differences were denoted via superscript ( a,b ) If superscripts are different (e.g. “a” vs “b”, then the groups are statistically different from each other within the given metric. Data are expressed as box plots.

Article Snippet: To confirm that that periostin protein was absent in Postn −/− samples, a rabbit polyclonal primary antibody for periostin (1:100, PAB16942, Abnova, Walnut, CA) was included.

Techniques: